Rapid identification of Salmonella using Hektoen enteric agar and 16s ribosomal DNA probe-gold nanoparticle immunochromatography assay in clinical faecal specimens
نویسندگان
چکیده
منابع مشابه
Isolation of Shigellae. 8. Comparison of xylose lysine deoxycholate agar, hektoen enteric agar, Salmonella-Shigella agar, and eosin methylene blue agar with stool specimens.
Two enrichment broths and four plating media were compared for efficiency of detection of enteric pathogens from 1,597 stool specimens. Of 170 salmonellae isolated from the composite of all methods, direct streaking yielded but 54%, whereas enrichment in gram-negative broth found 87% and Selenite-F broth 97%. By contrast, gram-negative broth produced 100% of the 17 shigellae, Selenite-F broth b...
متن کاملComparison of CHROMagar Salmonella medium and hektoen enteric agar for isolation of salmonellae from stool samples.
CHROMagar Salmonella (CAS), a new chromogenic medium, was retrospectively compared to Hektoen enteric agar (HEA) with 501 Salmonella stock isolates and was then prospectively compared to HEA for the detection and presumptive identification of Salmonella spp. with 508 stool samples before and after enrichment. All stock cultures (100%), including cultures of H2S-negative isolates, yielded typica...
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Citation: Wachiralurpan S, Sriyapai T, Areekit S, Sriyapai P, Augkarawaritsawong S, Santiwatanakul S and Chansiri K (2018) Rapid Colorimetric Assay for Detection of Listeria monocytogenes in Food Samples Using LAMP Formation of DNA Concatemers and Gold Nanoparticle-DNA Probe Complex. Front. Chem. 6:90. doi: 10.3389/fchem.2018.00090 Rapid Colorimetric Assay for Detection of Listeria monocytogene...
متن کاملA simple gold nanoparticle probes assay for identification of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex from clinical specimens.
We had previously developed a nested polymerase chain reaction (PCR)-immunochromatography test (ICT) for identification of Mycobacterium tuberculosis (MTB) and differentiation of MTB from other members of M. tuberculosis complex (MTBC) from clinical sputum samples (Soo P.C. et al., Journal of Microbiological Methods. 2006, 66(3):440-8.). To further improve the detection flexibility, simplicity ...
متن کاملComparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools.
Several chromogenic media have been developed to enhance the specificity of Salmonella detection. We compared the performance of four commercial chromogenic media-namely, ABC medium (Lab M. Ltd., Bury, United Kingdom), COMPASS Salmonella agar (Biokar Diagnostics, Beauvais, France), CHROMagar Salmonella agar (CHROMagar Company, Paris, France), and SM ID agar (bioMerieux, Marcy l'Etoile, France)-...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2013
ISSN: 0266-8254
DOI: 10.1111/lam.12191